The adsorption of intrinsic factor concentrate to glass: occurrence and prevention with reference to radioisotope dilution assays of vitamin B 12 .
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چکیده
منابع مشابه
Measurement of Serum Vitamin B12 Level Using Radioisotope Dilution and Coated Charcoal.
I N PREVIOUS REPORTS’3 it was shown that uncoated charcoal adsorbs both free vitamin B12 and B12 bound to proteins, but charcoal precoated by mixing with saturating quantities of albumin adsorbs only free B12. This finding has been confirmed.4 The charcoal particles may be considered as solid microsponges and the albumin coat as a molecular sieve surrounding each sponge. Small molecules such as...
متن کاملForms of vitamin B12 in radioisotope dilution assays.
Since the presence of analogues of vitamin B12 (B12, cobalamin, Cbl) has been postulated as the basis for the high values obtained by some radioisotope dilution assays (RIDA) of serum Cbl we examined serum for analogues. None could be demonstrated in the extracts of serum prepared for RIDA as sought by both direct and indirect techniques. The natural forms of serum Cbl were converted to cyanoco...
متن کاملPresence of cobalamin analogues in animal tissues.
Cobalamin (Cbl, vitamin B-12) has been extracted and isolated from a number of animal tissues by using (i) reverse-affinity chromatography on R protein-Sepharose followed by adsorption to and elution from charcoal-coated agarose and (ii) paper chromatography. Radioisotope dilution assays showed that only 75-97% of the Cbl chromatographed in the position of crystalline Cbl. The remaining 3-25% w...
متن کاملComparison of the radioisotope dilution-coated charcoal method and a microbiological method (L. leichmannii) for measuring vitamin B12 in serum.
A comparison has been made of the radioisotope dilution-coated charcoal method and a microbiological assay (with L. leichmannii as test organism) for determining the concentration of vitamin B(12) in serum. A satisfactory correlation was found between the results of the two methods. Under appropriate conditions the reproducibility of the radioisotope method compared favourably with that of the ...
متن کاملEffects of Vitamin B12 in Neonates and Young Infants
Vitamin B12 (cobalamin) is an essential coenzyme for nucleic acid synthesis. Animal protein is the major dietary source of vitamin B12. Deficiency of vitamin B12 leads to megaloblastic anemia, degeneration of the brain, spinal cord, peripheral nerves, and abnormalities of epithelial tissues. Two factors are necessary for the cure of megaloblastic anemia: one in food (extrinsic factor) and one i...
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ورودعنوان ژورنال:
- Blood
دوره 38 4 شماره
صفحات -
تاریخ انتشار 1971